Modelling of food transportation systems - a review

In 2002, over a million refrigerated road vehicles, 400,000 refrigerated containers and many thousands of other forms of refrigerated transport systems are used to distribute chilled and frozen foods throughout the world. All these transportation systems are expected to maintain the temperature of the food within close limits to ensure its optimum safety and high quality shelf life. Increasingly, modelling is being used to aid the design and optimisation of food refrigeration systems. Much of this effort has concentrated on the modelling of refrigeration processes that change the temperature of the food such as chilling, freezing and thawing. The purpose of a refrigerated transport system is to maintain the temperature of the food and appears to have attracted less attention from modellers. This paper reviews the work that has been carried out specifically on the modelling of food temperature, microbial growth and other parameters in the transportation of food. © 2006 Elsevier Ltd and IIR

Microbial contamination of food refrigeration equipment

Refrigeration systems in chilled rooms in 15 plants processing a variety of foods were studied. These included plants processing raw meat and salads, Chinese ready meals, dairy products, slicing and packing of cooked meats and catering establishments. An initial survey of total numbers of microbes at a total of 891 sites on evaporators, drip trays and chilled room walls was followed up with a more detailed examination of 336 sites with high counts, selecting for Listeria spp., coliforms, enterococci, Staphylococcus aureus and Bacillus cereus. Temperatures (particularly air on and air off, maximum and near defrost heaters) relative humidity, airflow, layout and cleaning regimes were surveyed. In general, no correlation could be found between any of the physical measurements and the numbers and types of bacteria detected. Maximum mean temperatures in the chilled rooms varied from -1 to +16.9 °C and few chilled units were regularly cleaned. Twenty five percent of sites examined had more than 105 colony-forming units per cm2, although, very few pathogens or faecal indicator bacteria were detected. Listeria spp. were not found and coliforms were found only once, in low numbers. Low numbers of S. aureus or B. cereus were present in 9 of the 15 plants, B. cereus was found on evaporators and associated drip trays in two catering plants and two plants processing cooked meat. Enterococci and S. aureus were found most frequently in a raw red meat slaughterhouse (always in low numbers). In general, microbial contamination was lower in rooms where wrapped rather than unwrapped products were stored. The type of product also affected the degree of contamination, with raw red meat and poultry or dry ingredients giving highest counts, and raw vegetables and cooked products lowest. The work demonstrated that bacteria were present on evaporator cooling coils in all factory cold rooms visited. Although evaporator-cleaning procedures were carried out in some factories as part of routine maintenance these were not shown to be effective at maintaining low levels of bacteria on evaporators. To maintain evaporator hygiene it is suggested that more regular cleaning procedures, possibly by means of automated cleansing systems, should be considered. © 2003 Elsevier Ltd. All rights reserved

Aging and aerobic fitness affect the contribution of noradrenergic sympathetic nerves to the rapid cutaneous vasodilator response to local heating

Sedentary aging results in a diminished rapid cutaneous vasodilator response to local heating. We investigated whether this diminished response was due to altered contributions of noradrenergic sympathetic nerves; assessing 1) the age-related decline and, 2) the effect of aerobic fitness. We measured skin blood flow (SkBF)(laser-Doppler flowmetry) in young (24±1 yr) and older (64±1 yr) endurance-trained and sedentary men (n=7 per group) at baseline and during 35 min of local skin heating to 42 °C at three forearm sites: 1) untreated; 2) bretylium tosylate (BT), preventing neurotransmitter release from noradrenergic sympathetic nerves; and 3) yohimbine and propranolol (YP), antagonising α- and β-adrenergic receptors. SkBF was converted to cutaneous vascular conductance (CVC) (SkBF/mean arterial pressure) and normalized to maximal CVC (%CVCmax) achieved by skin heating to 44 °C. Pharmacological agents were administered using microdialysis. In the young trained, the rapid vasodilator response was reduced at the BT and YP sites (P0.05) but treatment with BT did (P>0.05). Neither BT nor YP treatments affected the rapid vasodilator response in the older sedentary group (P>0.05). These data suggest that the age-related reduction in the rapid vasodilator response is due to an impairment of sympathetic-dependent mechanisms, which can be partly attenuated with habitual aerobic exercise. Rapid vasodilation involves noradrenergic neurotransmitters in young trained men, and non-adrenergic sympathetic cotransmitters (e.g., neuropeptide Y) in young sedentary and older trained men, possibly as a compensatory mechanism. Finally, in older sedentary men, the rapid vasodilation appears not to involve the sympathetic system

In search of future earths: Assessing the possibility of finding earth analogues in the later stages of their habitable lifetimes

© Copyright 2015, Mary Ann Liebert, Inc. Earth will become uninhabitable within 2-3 Gyr as a result of the increasing luminosity of the Sun changing the boundaries of the habitable zone (HZ). Predictions about the future of habitable conditions on Earth include declining species diversity and habitat extent, ocean loss, and changes to geochemical cycles. Testing these predictions is difficult, but the discovery of a planet that is an analogue to future Earth could provide the means to test them. This planet would need to have an Earth-like biosphere history and to be approaching the inner edge of the HZ at present. Here, we assess the possibility of finding such a planet and discuss the benefits of analyzing older Earths. Finding an old-Earth analogue in nearby star systems would be ideal, because this would allow for atmospheric characterization. Hence, as an illustrative example, G stars within 10pc of the Sun are assessed as potential old-Earth-analog hosts. Six of these represent good potential hosts. For each system, a hypothetical Earth analogue is placed at locations within the continuously habitable zone (CHZ) that would allow enough time for Earth-like biosphere development. Surface temperature evolution over the host star's main sequence lifetime (assessed by using a simple climate model) is used to determine whether the planet would be in the right stage of its late-habitable lifetime to exhibit detectable biosignatures. The best candidate, in terms of the chances of planet formation in the CHZ and of biosignature detection, is 61 Virginis. However, planet formation studies suggest that only a small fraction (0.36%) of G stars in the solar neighborhood could host an old-Earth analogue. If the development of Earth-like biospheres is rare, requiring a sequence of low-probability events to occur, biosphere evolution models suggest they are rarer still, with only thousands being present in the Galaxy as a whole

Probing Dark Matter

Recent novel observations have probed the baryonic fraction of the galactic dark matter that has eluded astronomers for decades. Late in 1993, the MACHO and EROS collaborations announced in this journal the detection of transient and achromatic brightenings of a handful of stars in the Large Magellanic Cloud that are best interpreted as gravitational microlensing by low-mass foreground objects (MACHOS). This tantalized astronomers, for it implied that the population of cool, compact objects these lenses represent could be the elusive dark matter of our galactic halo. A year later in 1994, Sackett et al. reported the discovery of a red halo in the galaxy NGC 5907 that seems to follow the inferred radial distribution of its dark matter. This suggested that dwarf stars could constitute its missing component. Since NGC 5907 is similar to the Milky Way in type and radius, some surmised that the solution of the galactic dark matter problem was an abundance of ordinary low-mass stars. Now Bahcall et al., using the Wide-Field Camera of the recently repaired Hubble Space Telescope, have dashed this hope.Comment: 3 pages, Plain TeX, no figures, published as a News and Views in Nature 373, 191 (1995

Development and evaluation of a diagnostic cytokine-release assay for Mycobacterium suricattae infection in meerkats (Suricata suricatta)

CITATION: Clarke, C., et al. 2017. Development and evaluation of a diagnostic cytokine-release assay for mycobacterium suricattae infection in meerkats (Suricata suricatta). BMC Veterinary Research, 13:2, doi:10.1186/s12917-016-0927-x.The original publication is available at http://bmcvetres.biomedcentral.comBackground: Sensitive diagnostic tools are necessary for the detection of Mycobacterium suricattae infection in meerkats (Suricata suricatta) in order to more clearly understand the epidemiology of tuberculosis and the ecological consequences of the disease in this species. We therefore aimed to develop a cytokine release assay to measure antigen-specific cell-mediated immune responses of meerkats. Results: Enzyme-linked immunosorbent assays (ELISAs) were evaluated for the detection of interferon-gamma (IFN-γ) and IFN-γ inducible protein 10 (IP-10) in meerkat plasma. An IP-10 ELISA was selected to measure the release of this cytokine in whole blood in response to Bovigam® PC-HP Stimulating Antigen, a commercial peptide pool of M. bovis antigens. Using this protocol, captive meerkats with no known M. suricattae exposure (n = 10) were tested and results were used to define a diagnostic cut off value (mean plus 2 standard deviations). This IP-10 release assay (IPRA) was then evaluated in free-living meerkats with known M. suricattae exposure, categorized as having either a low, moderate or high risk of infection with this pathogen. In each category, respectively, 24.7%, 27.3% and 82.4% of animals tested IPRA-positive. The odds of an animal testing positive was 14.0 times greater for animals with a high risk of M. suricattae infection compared to animals with a low risk. Conclusion: These results support the use of this assay as a measure of M. suricattae exposure in meerkat populations. Ongoing longitudinal studies aim to evaluate the value of the IPRA as a diagnostic test of M. suricattae infection in individual animals.http://bmcvetres.biomedcentral.com/articles/10.1186/s12917-016-0927-xPublisher's versio

The main and interactive effects of biological maturity and relative age on physical performance in elite youth soccer players

Abstract The main and interactive effect of biological maturity and relative age upon physical performance in adolescent male soccer players was considered. Consistent with previous research, it was hypothesised that participants of greater maturity or born earlier in the selection year would perform better in terms of physical performance tests. This cross-sectional study consisted of 84 male participants aged between 11.3 – 16.2 years from a professional soccer academy in the English Premier League. Date of birth, height, weight and parental height were collected. Sprint, change of direction, counter-movement jump and reactive strength index was considered for physical performance. Relative age was based on birth quarter for the selection year. Maturity status was based upon percentage of predicted adult height attained. Linear regression models highlighted that maturation was associated with performance on all but one of the physical performance tests, reactive strength index. In contrast, relative age only served as a significant predictor of performance on the countermovement jump. This study showed that physical performance (in the tests studied) seems to be related to the biological maturity status of a player but not their relative age. This finding is important because the paper suggests early maturing players perform better in the majority of physical performance tests, and the commonly held belief that relative age effect influences performance may be overstated